Atlantis and the seven-member crew left behind the station's new European Space Agency (ESA) Columbus laboratory and ESA astronaut Leopold Eyharts to prepare science experiments inside for operation.
After undocking, Atlantis Pilot Alan Poindexter completed a full fly around so his crewmates could obtain video and digital still imagery of Columbus docked to Harmony's right-side port.
The crew is performing a final inspection of the shuttle's thermal protection system in preparation for return to Earth. The Orbiter Boom Sensor System is used to search for any evidence of damage from orbital debris. That data will be reviewed by engineers over the next day.
Astronaut Daniel Tani, who has been in space since his launch to the space station in October, exercised to help prepare his body to feel the pull of gravity again.
Atlantis is targeted to land at the Kennedy Space Center Wednesday at 8:07 a.m. The weather forecast is favorable.
Today the crew is scheduled to go to sleep at 3:45 p.m. and awaken at 11:45 p.m.